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Biotin 抗体

This anti-Biotin antibody is a 山羊 多克隆 antibody detecting Biotin in WB, ELISA, IHC 和 DB. Suitable for .
Rockland
产品编号 ABIN349590
Supplier Product No.: 800-101-098
发货至: 中国

Quick Overview for Biotin 抗体 (ABIN349590)

抗原

Biotin

片段

Fab fragment

适用

请咨询

宿主

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山羊

克隆类型

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多克隆

标记

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This Biotin antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)
  • Supplier Product No.

    800-101-098

    Supplier

    Rockland

    原理

    Fab Biotin Antibody

    交叉反应 (详细)

    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.

    产品特性

    Synonyms: Goat Fab Anti-Biotin Antibody, Goat Fab Fragment Anti-Biotin Antibody

    纯化方法

    Fab fragment of Anti-Biotin antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads followed by papain digestion and chromatographic separation.

    过滤

    Sterile filtered

    免疫原

    Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

    Immunogen Type: Other

    亚型

    IgG
  • 应用备注

    Immunohistochemistry Dilution: User Optimized

    Application Note: Fab Anti-Biotin Antibody has been tested by ELISA and dot blot. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: User Optimized

    ELISA Dilution: 1:5,000

    Other: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    2.0 mg/mL

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

    有效期

    12 months
  • 抗原

    Biotin

    物质类

    Chemical

    背景

    Background: Fab Biotin antibody recognizes Biotin. Biotin, also known as vitamin H or coenzyme R, is a water-soluble B-vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis.
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